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. Author manuscript; available in PMC: 2016 Aug 18.
Published in final edited form as: Reprod Fertil Dev. 2015 Feb 18:10.1071/RD14375. doi: 10.1071/RD14375

Figure 2.

Figure 2

Validation of the IVFG system using two chemotherapeutics known to have adverse reproductive effects. The effects of increasing concentrations of CTX and CDDP on follicle (A, B) survival and (C, D) morphology during IVFG are shown. Follicles were treated with CTX and CDDP on day 2 of culture, and follicle survival and morphology were assessed on days 4 and 6. This experiment was repeated 4 times and a total of at least 60 follicles in each experimental group were examined. Representative images are shown. The arrowheads indicate the antral cavity, and the arrow indicates an oocyte that has been released from the follicle. Scale bar=100 μm. Statistically significant differences are denoted as follows: *P<0.05 and **P<0.001.