Fig. 2.

EICs of AKB-48 hydroxy metabolites in HLM and recombinant CYP incubations. AKB-48 was incubated with a HLM, b rCYP3A4, c rCYP2C19, and d rCYP2D6. Aliquots of the incubations were quenched and analyzed by LC-HR-MS. Representative EICs of AKB-48 (gray), AKB-48 monohydroxy (M1: green), dihydroxy (M2: blue), and trihydroxy (M3: yellow) metabolites are shown. The peaks are labelled according to Table I. The IS EIC is shown in red. The time of incubation was 45 min for HLM and 90 min for the recombinant enzymes