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. 2015 Apr 8;8(3):433–444. doi: 10.1007/s12195-015-0388-5

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© The Author(s) 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Characterization of PEG-PVX and RGD-PVX particles: (a) UV/Vis spectroscopy was used to determine number of dye molecules attached and to determine the concentration of PEG-PVX and RGD-PVX particles. (b) SDS-PAGE was used to confirm the covalent conjugation of PEG and RGD peptides to the PVX coat proteins (CPs). ImageJ software was used to determine the percentage of modified coat proteins. (c) Quantification of dye molecules and PEG/RGD ligands per PVX filament based on UV/Vis and SDS-PAGE. (d) Transmission electron microscopy was used to confirm the structural stability of PVX particles after modification (left to right: PVX, PEG-PVX and RGD-PVX). Scale bar = 100 nm.