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. 2015 Apr 8;8(3):433–444. doi: 10.1007/s12195-015-0388-5

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© The Author(s) 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Immunofluorescence analysis of tumor and lung sections. The tumor sections were stained with antibodies specific for the endothelial marker CD31 (red, a + b), integrin α _v [red, c + d (inset: higher magnification)], the tumor macrophage marker CD68 (pink, e + f) and DAPI (blue) to determine the localization of PEG-PVX and RGD-PVX within the tumor. (g–i) Whole lung (g) and lung sections (h, i) from mice treated with RGD-PVX were stained for α _v integrin (red) and CD68⁺ macrophages (pink). Co-localization analysis was used to highlight the hotspots of RGD-PVX accumulation with integrins and CD68 macrophages (i). Scale bars = 50 μm.