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. 2015 May 15;57(9):801–813. doi: 10.1007/s12033-015-9873-2

Fig. 3.

Fig. 3

The optimization of reaction conditions of PNA-mediated PCR clamping. The specific inhibition of ERVWE1 by an assay relies on exact reciprocal probe-to-primer localization and on the optimal concentration of the PNA. The best results were obtained if a PNA probe shared only one base with the adjacent reverse primer (sync_1_REV/-1)