Fig. 5.

The quantitative analysis of MSRV mRNA in cell lines. An amount of MSRV mRNA was estimated in normal human astrocytes (NHA) and in human umbilical vein endothelial cells (HUVECs) in in vitro cultures. The addition of PNA into the reaction increased the calculated MSRV copy number in both NHA and HUVECs when compared with the quantitative analysis without the use of PNA. These results indicate that an inhibitory effect caused by ERVWE1 present in the sample may be eliminated by the use of ERVWE1-specific PNA and that ERVWE1 is more abundantly expressed in NHA than in HUVECs (1.86- and 0.67-fold increase in calculated mRNA copies number after addition of PNA, respectively). For the quantitative analysis of MSRV in cells, an individual, in-run standard curve was generated in the range from 1 × 10 e⁸ copies/reaction to 1 × 10 e² copies/reaction (dotted line with data labels)