Table 1.

Oligonucleotide primers used in the work—a summary

Oligonucleotide name	Sequence	Remarks
Syncytin_out_F	5′-TGCCCCATCGTATAGGAGTC-3′	For the cloning procedure, PNA-mediated PCR and megaprimer mutagenesis
Syncytin_out_R	5′-CGGGTGAGTTGGGAGATTAC-3′	For the cloning procedure
sync1_REV/-2*)	5′-ATTCCACCCCCATCAGACATA-3′	*) Reverse primers used in the PNA-mediated PCR clamping together with Syncytin_out_F as forward primer
sync1_REV/-1*)	5′-AATTCCACCCCCATCAGACAT-3′	As above
sync1_REV/0*)	5′-GAATTCCACCCCCATCAGACA-3′	As above
Mutagenic MRSV	5′-CAGACATACTGGTATGGGTGAAGT-3′	For use in the megaprimer mutagenesis technique
PNA ERVWE1	N′-TACCAGTTTGGGTG-C′	For the PNA-mediated PCR clamping of ERVWE1
PNA ERVWE1-T	N’-TACCAGTTTGGGTGA-C’	For the PNA-mediated PCR clamping of ERVWE1
MSRV Probe	FAM 5′-CTTACTTCACCCATACCAGTATGTCTGATG- 3′ BHQ1	For the quantitation of MSRV sequences by means of QPCR