Fig. 3.

SDS-PAGE analysis of purified cgAUS. About 4 µg total protein was loaded on each lane. Lane-numbers in a and b correspond to the described cgAUS samples within this article. Lane-number 1–4: active cgAUS; lane-number 5: latent cgAUS; lane-numbers 6 and 7: proteolytically cleaved latent cgAUS; M, molecular weight marker. a SDS-PAGE (according to Laemmli 1970) of purified cgAUS. b SDS-PAGE of purified cgAUS under semi-denaturating conditions: Laemmli sample buffer without DTT, samples were not boiled