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. 2015 Feb 20;242(3):519–537. doi: 10.1007/s00425-015-2261-0

Table 1.

Yields, molecular masses (SDS-PAGE) and the kinetic classification of purified cgAUS samples. 6 kg frozen petal material was used as starting material

Sample Amount (mg) M (kDa) (reducing SDS-PAGE) M (kDa) (non-reducing SDS-PAGE) Latent/active
1 2.45 37; 38 36; 37; 38 Active
2 0.56 37; 38 36; 37; 38 Active
3 0.37 37; 38 36; 37; 38 Active
4 1.25 37; 38 36; 37; 38 Active
5 0.87 59 65, 61, 54 Latent
6 0.25 38; 19 65, 54 Latent
7 0.12 37; 19 65, 54 Latent

Molecular masses were determined by reducing and non-reducing SDS-PAGE. Satellite bands exhibiting a shift to lower masses are found due to incomplete denaturation and/or incomplete reduction of the applied samples