Table 1.
Sample | Amount (mg) | M (kDa) (reducing SDS-PAGE) | M (kDa) (non-reducing SDS-PAGE) | Latent/active |
---|---|---|---|---|
1 | 2.45 | 37; 38 | 36; 37; 38 | Active |
2 | 0.56 | 37; 38 | 36; 37; 38 | Active |
3 | 0.37 | 37; 38 | 36; 37; 38 | Active |
4 | 1.25 | 37; 38 | 36; 37; 38 | Active |
5 | 0.87 | 59 | 65, 61, 54 | Latent |
6 | 0.25 | 38; 19 | 65, 54 | Latent |
7 | 0.12 | 37; 19 | 65, 54 | Latent |
Molecular masses were determined by reducing and non-reducing SDS-PAGE. Satellite bands exhibiting a shift to lower masses are found due to incomplete denaturation and/or incomplete reduction of the applied samples