Fig. 5.

Impact of IL-1β, TNF-α and APS on COX2 mRNA (a, b) and protein expression (c) in SZ95 sebocytes. Expression of COX2 transcripts was determined by real-time RT-PCR following stimulation of cells with either IL-1β or TNF-α (10 ng/ml) alone or in combination with APS for 8 h. *p < 0.05 vs. control for IL-1β, n = 3; *p < 0.05 vs. TNF-α, n = 4. Expression of COX2 protein was assessed by Western immunoblotting following stimulation of cells with IL-1β or TNF-α as indicated. Expression of COX2 protein was quantified by densitometry. Panels are representative images of at least three independent experiments with identical results