Table 1.
Primary antibodies
| Antibody | Species, isotype | Company | Dilution |
|---|---|---|---|
| GFP | Chicken | Abcam | 1:1000 |
| HB9 | Mouse, IgG1 | DSHB | 1:100 |
| NKX6.1 | Mouse, IgG1 | DSHB | 1:100 |
| ISL1/2 | Mouse, IgG2b | DSHB | 1:100 |
| LIM3 | Mouse, IgG1 | DSHB | 1:100 |
| CHAT | Goat | Chemicon | 1:100 |
| TUJ1 | Mouse or rabbit | Covance | 1:500 |
| MAP2 | Mouse | Sigma-Aldrich | 1:200 |
| PAX6 | Rabbit | Covance | 1:75 |
| SMI32 | Mouse | Millipore | 1:1000 |
| OLIG2 | Rabbit | Millipore | 1:200 |
| FOXA2 | Goat | Santa Cruz Biotechnology | 1:100 |
| GLI3 | Rabbit | Wang et al., 2000 | 1:100 |
DSHB, Developmental Studies Hybridoma Bank.