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. 2015 Aug 19;35(33):11543–11558. doi: 10.1523/JNEUROSCI.5267-14.2015

Figure 4.

Figure 4.

HGF/MET signaling did not increase ENS precursor migration in culture. A–C, E12.5 gut slices were cultured 24 h on fibronectin-coated dishes with no added factor, HGF, or GDNF before staining for RET and TuJ1. A, ENCDCs migrate from the gut slice onto the culture dish even without any added factors. B, HGF did not increase the distance that ENCDCs migrated from the edge of the gut slice. C, GDNF markedly increased the distance ENCDCs migrate from the edge of the gut slice. D, Quantitative data (no added factor, 15 slices; 50 ng/ml HGF, 14 slices; 100 ng/ml HGF, 19 slices; 100 ng/ml GDNF, 30 slices; N = 3 independent experiments). *p < 0.001 for GDNF versus no added factor, ANOVA with Dunn's multiple-comparison test.