TABLE 4.
Performance of Aspergillus PCR when testing serum and plasma samples
| Parametera | Serum |
Plasma |
||
|---|---|---|---|---|
| Single PCR positive threshold | Multiple (≥2) PCR positive threshold | Single PCR positive threshold | Multiple (≥2) PCR positive threshold | |
| Sensitivity (no./total no., % [95% CI]) | 13/19, 68.4 (46.0–84.6) | 8/19, 42.1 (23.1–63.7) | 18/19, 94.7 (75.4–99.1) | 13/19, 68.4 (46.0–84.6) |
| Specificity (no./total no., % [95% CI]) | 32/42, 76.2 (61.5–86.5) | 40/42, 95.2 (84.2–98.7) | 35/42, 83.3 (69.4–91.7) | 42/42, 100 (85.1–100) |
| Positive LR | 2.9 | 8.8 | 5.7 | >68.4a |
| Negative LR | 0.4 | 0.6 | 0.06 | 0.32 |
| DOR | 7.5 | 14.7 | 95.0 | >213.8a |
a
95% CI, 95% confidence interval; LR, likelihood ratio; DOR, diagnostic odds ratio. Positive LR and DOR values were calculated with a specificity of 99% to overcome ∞ that would be generated with 100% specificity.