TABLE 1.
Results of FilmArray BioThreat E panel and qRT-PCR testing using serial dilutions of inactivated Zaire ebolavirus at known titers in whole blooda
| Virus titer (TCID50/ml) | NP2 results |
VP40 results |
FilmArray results |
|||
|---|---|---|---|---|---|---|
| Test 1 | Test 2 | Test 1 | Test 2 | Test 1 | Test 2 | |
| 40,000,000 | Positive (CT = 20) | Positive (CT = 20) | Positive (CT = 18) | Positive (CT = 18) | Detected | Detected |
| 4,000,000 | Positive (CT = 23) | Positive (CT = 23) | Positive (CT = 22) | Positive (CT = 22) | Detected | Detected |
| 400,000 | Positive (CT = 26) | Positive (CT = 26) | Positive (CT = 25) | Positive (CT = 25) | Detected | Detected |
| 40,000 | Positive (CT = 30) | Positive (CT = 30) | Positive (CT = 28) | Positive (CT = 28) | Detected | Detected |
| 4,000 | Positive (CT = 33) | Positive (CT = 33) | Positive (CT = 32) | Positive (CT = 32) | Detected | Detected |
| 400 | Positive (CT = 37) | Positive (CT = 35) | Positive (CT = 34) | Positive (CT = 34) | Detected | Detected |
| 40 | Negative | Negative | Negative | Positive (CT = 37) | Not detected | Not detected |
| 0 | Negative | Negative | Negative | Negative | Not detected | Not detected |
a
Zaire ebolavirus (strain 812592) stock at a known titer of 4 × 108 TCID50/ml was inactivated by gamma irradiation prior to testing. EUA-approved NP2 and VP40 qRT-PCR assays and FilmArray BT-E panel testing were performed in duplicate, using 10-fold serial dilutions of inactivated virus in whole blood, at the CDC.