TABLE 2.

Aspergillus PCR performance using plasma samples spiked with different fungal loads^a

Sample no.	Burden (ge/ml)	95% sample variance	Positivity rate (%)	Mean Cq (95% CI)	SE
1	1,000	939.6–1,060.4	100	32.4 (30.7–34.0)	0.70
2	100	80.8–119.2	100	36.1 (34.1–38.1)	0.84
3	50	36.4–53.6	100	36.9 (35.1–38.7)	0.75
4	10	4.0–16.0	100	39.00 (37.1–40.9)	0.79
5	5	0.8–9.2	100	39.9 (37.8–42.0)	0.88
6	1	0–2.92	8.3	40.0b	NAc
7, 8	0 ×2	NA	0	No signal	NA

^aPlasma samples 1 to 8 were spiked with different fungal burdens (genome equivalents [ge]/ml of plasma).

^bPlasma 6 was positive in one center in a single sample only.

^cNA, not available.