TABLE 3.

Influence of sample volume (0.1 to 0.5 ml) on Aspergillus real-time PCR crossing points when testing plasma samples containing a range of A. fumigatus genomic DNA concentrations (5 to 1,000 ge/ml)^a

Plasma vol (ml)	Fungal load (ge/ml of plasma)
	5	10	50	100	1,000
0.1	40.68	39.70	37.43	36.45	33.21
0.2	39.90	38.92	36.65	35.67	32.42
0.3	39.11	38.14	35.86	34.89	31.64
0.4	38.33	37.35	35.08	34.11	30.86
0.5	37.55	36.57	34.30	33.32	30.07

^aThe prediction of C_q values was calculated using a linear mixed model.