TABLE 4.

Details of the molecular procedures used by the EAPCRI centers investigating the performance of Aspergillus PCR when testing serum and plasma

EAPCRI center no.	No. of serum samples/mo	DNA extraction vol (ml)	DNA extraction system	Vol (μl) of:			PCR target gene	Sensitivity (%) in:
				Elution	Template	PCR		Serum	Plasma
1	0	0.2	MagNA Pure	50	10	20	18S	66.7	100
2	80	1	QIAamp UltraSens virus	70	10	21	ITS	100	100
3	40	1	QIAamp UltraSens virus	38	10	21	ITS	100	100
4	15	0.2	QIAamp DNA mini	50	2	20	ITS	0	100
5	70	1	MagNA Pure total NA large volume	50	10	20	18S	100	100
6	240	0.5	EZ1 DSP virus	60	15	50	28S	100	100
7	50	0.5	MagNA Pure total NA large volume	100	5	20	28S	100	100
8	10	0.5	MagNA Pure viral NA large volume	50	10	50	28S	100	100