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. 2015 May 28;32(9):2417–2432. doi: 10.1093/molbev/msv125

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© The Author 2015. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Fig. 6. — het-e functions as a het locus in Neurospora crassa, and which is dependent upon vib-1. (A) Strains with compatible and incompatible het-e alleles were used to forced heterokaryons using auxotrophic markers. The recipient strain for constructing strains carrying het-e2 or het-e3 alleles was a Δhet-e1 mutant derived from the sequenced strain FGSC 2489. (A1) A forced heterokaryon (his-3::Pccg1-het-e1-gfp; pan-2 + Δhet-e1; his-3; pyr-4) showed a compatible growth phenotype indistinguishable from a WT homokaryon. (A2) A forced heterokaryon carrying different het- e alleles (het-e1 and het-e3) (het-e1; his-3 + his3::Pccg1-het-e3-gfp; pan-2) showed a typical incompatible phenotype of inhibited growth. (A3) A forced heterokaryon (his3::Pccg1-het-e2-gfp; pan-2 + Δhet-e1; his-3; pyr-4) showed a compatible phenotype. (A4) A forced heterokaryon between strains carrying different het-e alleles (het-1 and het-e2) (het-e1; his-3 + his3::Pccg1-het-e2-gfp; pan-2) showed an incompatible phenotype. (A5) A forced heterokaryon (his3::Pccg1-het-e3-gfp; pan-2 + Δhet-e1; his-3; pyr-4) showed a compatible phenotype. (A6) A forced heterokaryon between strains carrying different het-e alleles (het-e2 and het-e3) (his-3::Pqa2-het-e2; pyr-4 + his3::Pccg1-het-e3-gfp; pan-2) showed an incompatible phenotype. (B) het-e mediated heterokaryon incompatibility is suppressed by deletions in vib-1. (B1) A forced heterokaryon with identical het-e alleles (his-3::Pccg1-het-e1-gfp; thr-2 + het-e1; pan-2) showed a compatible phenotype. (B2) A forced heterokaryon between strains that have different het-e alleles (het-e1 and het-e2) (het-e1; pan-2 + his3::Pccg1-het-e2-gfp; thr-2) showed a typical incompatible phenotype. (B3) A forced heterokaryon between strains with identical het-e (het-e1) alleles in a homozygous vib-1 deletion background (his-3::Pccg1-het-e1-gfp; Δvib-1; thr-2 + het-e1; Δvib-1; pan-2) showed a compatible phenotype. (B4) A forced heterokaryon between strains carrying different het-e alleles (het-e1 and het-e2) in vib-1 deletion background (his3::Pccg1-het-e2-gfp; Δvib-1; thr-2 + het-e1; Δvib-1; pan-2) showed a compatible phenotype. (C) RT-PCR showing het-e expression levels in a WT strain (FGSC 2489) versus a vib-1 deletion strain. The expression level of het-e in FGSC 2489 was set arbitrarily to one. Error bars indicate standard deviation.