Table 3.
Results of preliminary phytochemical analysis in aqueous crude extracts of Artemisia afra aerial part.
| Secondary metabolite | Reagents and method | Indicators | Artemisia afra |
|---|---|---|---|
| Chromophores | 10 mL distilled water + heat for 30 min | Yellow to red color | +++ |
| Polyphenols | 1% FeCl3 + 1 mL of K3Fe (CN)6 | Green blue color | − − − |
| Saponins | 30 mL distilled water + heat (5 min) | Formation of honey comb froth | +++ |
| Phytosteroids and withanoids | CHCl3 + conc. H2SO4 | Red, reddish brown, or violet color | +++ |
| Flavonoids | 5 drops of 2% lead acetate | Yellow or orange color | +++ |
| Tannins | 3 drops of 1% K3[Fe(CN)6] + 3 drops of conc. NH3 | Formation of color | +++ |
|
| |||
| Anthraquinone glycosides | 2 N HCL, benzene, 10% ammonia | Red color | +++ |