Fig. 4.

Impact on EKLF and FLI-1 levels after introduction of Tpo to an Epo-induced culture. UT-7/GM cells were starved of growth factor and subsequently passaged into medium with only Epo or Epo+Tpo. On day 3, part of the culture in Epo-only medium was passaged into Epo+Tpo medium. On day 6, cells that were switched from Epo-only to Epo+Tpo medium exhibited significantly higher EKLF transcript expression [*median 33% higher, 95% confidence interval (11%, 59%)], but similar FLI-1 transcript expression [**median 3% higher, 95% confidence interval (−10%, 20%)] when compared to cells maintained in Epo+Tpo medium throughout. The box represents the 25th–75th percentiles, and the median is indicated. For each box, the interquartile range (IQR) is defined as the difference between the 75th and 25th percentile values; the top whisker is at 1.5× IQR above the 75th percentile, and the bottom whisker is at 1.5× IQR below the 25th percentile or zero, whichever is greater.