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. 2015 Jul 31;4:e08942. doi: 10.7554/eLife.08942

Figure 6. Resection mutants fail to reduce transcription around the DSB.

Figure 6.

(A) DSB resection. YEPR exponentially growing cell cultures of JKM139 derivative strains, carrying the HO cut site at the MAT locus, were transferred to YEPRG at T0. Formation of ssDNA was determined as described in Figure 5B. (B) Binding of Rpb2-HA in wild type cells from samples collected in (A) was evaluated by ChIP and qPCR as described in Figure 2B. The mean values ±s.d. are represented (n = 3). (C) Binding of Rpb2-HA in exo1∆ sgs1∆ cells from samples collected in (A) was evaluated by ChIP and qPCR as described in Figure 2B. The mean values ±s.d. are represented (n = 3). (D) RNA levels from samples collected in (A) were analyzed at T0 and T240 after HO induction by qRT-PCR as described in Figure 2A. The mean values ±s.d. are represented (n = 3). Contiguous images that were used for the cropped final Figure 6A are available in Figure 6—source data 1.

DOI: http://dx.doi.org/10.7554/eLife.08942.010

Figure 6—source data 1. Contiguous images that were used for the cropped final Figure 6A.
DOI: http://dx.doi.org/10.7554/eLife.08942.011
elife08942s003.tif (2.3MB, tif)
DOI: 10.7554/eLife.08942.011