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. 2014 Sep;93(9):891–897. doi: 10.1177/0022034514543015

Figure 2.

Figure 2.

A novel isoform of periostin isolated from human PDL cells shows specific expression in PDL tissue. (A) Periostin isoforms isolated from human PDL cells. RT-PCR analysis of the 3′-region of periostin generated multiple amplicons. This illustration of the C-terminal region of isolated periostin isoforms shows exon-intron arrangement, expression frequency, and numbers of amino acids. MW, molecular weight; HPDL, human periodontal ligament cells; RD1, repeated domain 1; RD2, repeated domain 2; RD3, repeated domain 3; RD4, repeated domain 4; Frequency, expression frequency in the cDNA profile; AA, numbers of amino acids in the isoform. Isoform-specific RT-PCR analysis of periostin in mRNA from the indicated tissues (B) and human oral-tissue-derived cell lines (C). RT-PCR primers annealing to exons a and c′ were used. The size of each amplified isoform is indicated on the right of the gel image. The number of PCR cycles is shown above each lane in (B). The number of PCR cycles in (C) is 30. HPRT, hypoxanthine phosphoribosyl transferase. Results shown are representative of three independent experiments.