Figure 5.

Release of ATP from cultured human odontoblast-like cells. (a) Changing the cell culture medium caused an increase in extracellular ATP concentration 1 and 5 min afterward, which returned to basal levels by 15 min. (b) 100nM GSK1016790A, (d) 500µM allyl isothiocyanate (AITC), and (f) 500µM CA caused a significant increase in extracellular ATP concentration, measured 15 min after agonist administration. These increases were significantly inhibited by pretreatment with (c) 10µM HC067047 or (e, g) 100µM AP18. ^*p < .05, ^**p < .01 vs. vehicle/basal groups. ^#p < .05, ^##p < .01 vs. agonist + antagonist vehicle groups. n = 8-10 groups of paired duplicate measurements.