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. 2015 Jul 1;114(2):1146–1157. doi: 10.1152/jn.00355.2015

Fig. 2.

Fig. 2.

pigk mRNA is maternally expressed and detected during embryonic stages of development. To test for the presence of pigk mRNA during embryonic development, we performed qPCR using RNA extracted from wild-type (WT) embryos, touch-unresponsive maco embryos, and their responsive siblings. Data are presented as means ± SD, with pigk mRNA levels normalized to those of actb2 mRNA (see materials and methods). A: qPCR detected pigk mRNA at the one-cell stage, indicating that the transcripts are maternally provided. B: in WT embryos, pigk expression levels increased between 22 and 48 h postfertilization (hpf). *P < 0.05, **P < 0.01. C: both responsive (maco+/+ and macott261/+, pooled) and unresponsive (macott261/tt261) sibling embryos expressed pigk mRNA at 36 and 48 hpf. At both time points, unresponsive and responsive siblings had similar pigk expression levels.