Fig. 4.
Ubiquitous but not RB-restricted expression of WT pigk rescues touch responsiveness in macott261/tt261 embryos. One-cell-stage embryos were either not injected or injected with the indicated transgenic constructs. Touch responsiveness was assayed at 48 hpf, and genotyping was performed after behavioral testing. In the graphs, the solid horizontal lines designate the median score, boxes indicate the 25th–75th percentile range, and whiskers show the 90th percentile. Sample size (n) is indicated within the figure. The data presented in A for uninjected embryos are reshown in B and C as the uninjected conditions. A: uninjected WT (maco+/+; column 1) and heterozygote (macott261/+; column 2) embryos have significantly higher touch scores than do sibling macott261/tt261 mutants (column 3). ***P < 0.001. B: data were pooled for maco+/+ and macott261/+ embryos injected with the same transgene. Regardless of transgene injection, the different sibling groups all had touch scores that were closer to 10 than 0. However, sibling embryos injected with ubb:pigk-IRES-EGFP (column 2) had slightly higher median scores than those injected with ubb:pigk_M1T-IRES-eGFP (column 3) or either of the CREST3 driven transgenes (columns 4 and 5). *P < 0.05, ***P < 0.001. C: data for embryos that were genotypically homozygous for the maco mutation (macott261/tt261). macott261/tt261 embryos injected with ubb:pigk-IRES-EGFP (column 2) had significantly greater touch scores than did uninjected macott261/tt261 embyros (column 1) or those injected with ubb:pigk_M1T-IRES-EGFP (column 3), CREST3:pigk-IRES-EGFP (column 4), or CREST3:pigk_M1T-IRES-EGFP (column 5). *P < 0.05, ***P < 0.001.