Figure 1.

Microfluidic chambers can be used to study compartmental localization of the effects of acetylation inhibitors and other neuronal changes in adult axons.

Microfluidic chambers can be cast by pouring silicone elastomer into a prefabricated microfluidic template and holes can be punched to mark the chambers. Image below shows magnification of microfluidic channels on both sides of the central chamber. Microfluidic chambers are placed over a chondroitin sulfate proteoglycan border stripe before neurons are plated into a small hole and allowed to occupy the central chamber (cell body chamber). Neurons can be grown for several days until axons reach the chondroitin sulfate proteoglycan (CSPG) border stripe.