Figure 2.

Reference batch cultivations of AF1000 pJBGT3RX with depletion of either ammonium (A) or phosphate (B) for production of 3HB. The measured parameters were: cell dry weight (CDW, filled circles), glucose (Glc, crosses), ammonium (NH${}_4^{+}$, filled squares), phosphate (PO${}_4^{3-}$, open squares), acetic acid (HAc, filled triangles), and (R)-3-hydroxybutyrate (3HB, open triangles). Specific rates (μ, q_3HB, q_Hac, and q_Glc) are represented as functions obtained from least square fits of the data. All parameters except ammonium, phosphate and CDW (B) were fit with first order polynomials. CDW (B) was fit with a second order polynomial.