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. 2015 Aug 19;6:844. doi: 10.3389/fmicb.2015.00844

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Copyright © 2015 Guevara-Martínez, Sjöberg Gällnö, Sjöberg, Jarmander, Perez-Zabaleta, Quillaguamán and Larsson.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Ammonium-limited fed-batch cultivation of AF1000 pJBGT3RX for production of 3HB. The measured parameters were: cell dry weight (CDW, filled circles), glucose (Glc, crosses), ammonium (NH $_{4}^{+}$ , filled squares), acetic acid (HAc, filled triangles) and (R)-3-hydroxybutyrate (3HB, open triangles). Specific rates (μ, q_3HB, q_Hac, q_Glc, and q_CO2) are represented as functions obtained from least square fits of the data and online measurements. CDW and 3HB have been fit with an exponential function in the exponential feed phase and a first order polynomial in the linear feed phase. HAc was fit with a first order polynomial in both phases.