Fig. 1.

Regulation of CCL2 production by the accumulation of endogenous p53. a A549 cells were transfected with 0.5 μg reporter DNA (mcl2pwt) for 2 h and exposed to UV radiation. Cells were then cultured. The treated cells were harvested at different times (0, 1, 2, 4, 6, 8, 16, or 24 h), and the lysate from cells at each time point was purified and analyzed by Western blot with antibodies against p53, luciferase, or actin as control. b Regulation of CCL2 promoter activity by overexpression of p53. A549 cells were transfected with mcl2pwt DNA alone or co-transfected with 0.5 μg mcl2pwt DNA and different concentrations (0, 0.1, 0.25, 0.5, or 1 μg) of pcp53WT DNA. Cells were cultured overnight. The lysate from cells in each group was assessed by luciferase assay (n = 3). Data are presented as mean ± SEM