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. Author manuscript; available in PMC: 2015 Oct 1.
Published in final edited form as: Tumour Biol. 2014 Dec 11;36(4):2801–2808. doi: 10.1007/s13277-014-2906-9

Fig. 2.

Fig. 2

Analysis of CCL2 sequence. Mouse CCL2 5′UTR&promoter sequence from −555 to +85. BLAST search of the sequence showed that it was identical to mouse genomic DNA (AL713839) and ended before the start codon of CCL2. The putative binding sites for p53 in CCL2 5′UTR&promoter were analyzed by PROMO version 3.0.2 (UPC) and displayed in bold characters. The binding sites for other potential transcription factors have not been analyzed. The oligonucleotide, including p53 binding site (underlined sequence, named CCl2/p53oligo) and its complementary oligo, was synthesized. Both synthetic oligonucleotides were annealed and labeled with [32P]ATP as a double-stranded DNA probe for EMSA