. Author manuscript; available in PMC: 2015 Oct 1.

Published in final edited form as: Tumour Biol. 2014 Dec 11;36(4):2801–2808. doi: 10.1007/s13277-014-2906-9

Table 1.

Primer pairs used for PCR of DNA constructs

DNA clone		1^st PCR amplification	2^nd PCR amplification
No.	Name	Primer pair	Primer pair
1	Pcp 53wt	5′-ATGACTGCCATGGAGGAGTC-3′+5′-TCAGTCT GAGTCAGGCCCCA-3′
2	pcCCL2	5′-CTCGAGCCATGCAGGTCCCTGTCATG-3′+5′- AAGCTTCTAGTTCACTGTCACACTG-3′
3	mcL2pwt	5′-ACTCAGACAGTTCATATCAA-3′+5′-GGTGGT GGAGGAAGAGAGAGAG-3′
4	mcl2p115	5′-CAACTTCCACTTTCCATCAC-3′+5′-GGTGGTG GAGGAAGAGAGAGAG-3′
5	mcl2p315	5′-GCAGAGCCACTCCATTCACA-3′+5′-GGTGGTG GAGGAAGAGAGAG-3′
6	mcl2p53m	5′-CAACTTCCACTTTCCATCACTTATCCAGGGTG ATGCTACTCCTTGGCACCAAGCACCCTGCCT GACTCCACCCCCCTGGCTTACAATAAAAGGC TGCCTC-3′+5′-GGTGGTGGAGGAAGAGAGAG CTGGCTTCAGTGAGAGTTGGCTGGTGCTGGC GTCTGGCTCTCTGCACTTCTGGCTGCTCTGAG GCAGCCTTTTATTGTAA-3′	5′-CAACTTCCACTTTCCATCAC-3′+5′- GGTGGTGGAGGAAGAGAGAG-3′