Figure 2.

HIV-1 protein-induced IL-8 stimulation was significantly increased in TLR2 transgene cell lines. (A) TZMbl and TZMbl-2 cells, (B) primary CD3 + T cells were treated with non-specific (N.S.) or anti-TLR2 (α-TLR2) antibody for 1 h prior to treatment with 500 ng/mL TLR2 ligand (Pam₃CSK₄), 1 μg/mL HIV-1 proteins (p17, p24, gp41, gp120) or medium (control) and supernatants were collected after 4 h. (C) HEK293-TLR2/1 cells and (D) HEK293-TLR2/6 cells were treated overnight with 500 ng/mL TLR2 ligand (Pam₃CSK₄), 1 μg/mL HIV-1 proteins (p17, p24, gp41, and gp120), or medium (control) supernatants were evaluated for IL-8 production with ELISAs. Data are the mean of triplicate samples ±SEM. *P < 0.05, **P < 0.01, ***P < 0.001. Inserts: mRNA expression of TLR1, TLR2, TLR3, TLR4, TLR6, TLR10, and β-actin was detected in the cell lines with conventional RT-PCR as shown by electrophoresis agarose gels. A representative data set of at least three independent experiments is shown.