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. 2015 Aug 21;21(31):9317–9327. doi: 10.3748/wjg.v21.i31.9317

Table 1.

Oligonucleotide primers used for polymerase chain reaction

Gene Primer Sequence (5'→3') PCR products (bp) Annealing temperature (°C) Ref.
16S rRNA HP1 GCAATCAGCGTCAGTAATGTT C 519 58.5 [85]
HP2 GCTAAGAGATCAGCCTATGTCC
vacA (s1, s2) VA1F ATGGAAATACAAGAAACACACC s1: 259 56.0 [25]
VA1R CTGCTTGAATGCGCCAAACTTTAATC s2: 286
vacA (m1, m2) VAG-F CAATCTGTCCAATCAAGCGAG m1: 570 58.5 [25]
VAG-R GCGTCAAAATAATTCCAAGG m2: 645
cagA D008 ATAATGCTAAATTAGACAACTTGAGCGA 298 58.5 [86]
R008 TTAGAATAATCAACAAACATCACGCCAT

PCR: Polymerase chain reaction.