Table 1.
Gene | Primer | Sequence (5'→3') | PCR products (bp) | Annealing temperature (°C) | Ref. |
16S rRNA | HP1 | GCAATCAGCGTCAGTAATGTT C | 519 | 58.5 | [85] |
HP2 | GCTAAGAGATCAGCCTATGTCC | ||||
vacA (s1, s2) | VA1F | ATGGAAATACAAGAAACACACC | s1: 259 | 56.0 | [25] |
VA1R | CTGCTTGAATGCGCCAAACTTTAATC | s2: 286 | |||
vacA (m1, m2) | VAG-F | CAATCTGTCCAATCAAGCGAG | m1: 570 | 58.5 | [25] |
VAG-R | GCGTCAAAATAATTCCAAGG | m2: 645 | |||
cagA | D008 | ATAATGCTAAATTAGACAACTTGAGCGA | 298 | 58.5 | [86] |
R008 | TTAGAATAATCAACAAACATCACGCCAT |
PCR: Polymerase chain reaction.