| Signal | Green Fluorescence | Red Fluorescence I | Red Fluorescence II | Luminescence |
| Protein or dye | Green Fluorescence Protein (GFP) | mCherry protein or Nile Red rhamnolipid stain | SYTO® 64 | Luciferase from lux operon |
| Excitation wavelength (nm) | 480 ± 10 | 540 ± 10 | 590 ± 10 | Off |
| Emission wavelength (nm) | 535 ± 17.5 | 600 ± 17.5 | 670 ± 17.5 | No filter |
| Exposure time (sec) | 30 | 60 | 60 | 240 |
| f-stop | 4.0 | 4.0 | 2.5 | 1.1 |
| FOV (mm) | 190 | 190 | 140 | 120 |
| Focal plane (mm) | 27.5 | 27.5 | 12.2 | 4 |
| Binning (pixels) | None | 2 x 2 | None | 8 x 8 |
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