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. 2014 Dec 23;55(12):8402–8408. doi: 10.1167/iovs.14-14992

Figure 1.

Figure 1

Western blot analysis of eNOS, eNOS-phospho Ser1177, eNOS-phospho Thr495, and Akt-phospho levels in cultured AAP cells. (A) Representative Western blots showing a comparison of protein/phosphorylation levels in AAP cell monolayers cultured under normal or hyperoxic conditions for 2 weeks (aged). (B–D) Densitometric analyses of Western blots for eNOS-phospho Ser1177 (normalized to total eNOS), eNOS-phospho Thr495 (normalized to total eNOS), or Akt-phospho (normalized to glyceraldehyde 3-phosphate dehydrogenase) from control and aged cells. *P < 0.05; n = 6. Error bar denotes SD.