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Effect of aging on nitrite concentration in AAP cells exposed to shear stress. The NO concentration was measurements by the Griess assay (Sigma; Sigma-Aldrich Corp.). Shown are combined data from four experiments (mean ± SD) showing the nitrite content in AAP cells (control versus aged) exposed to shear stress (10 dynes/cm2 for 24 hours) compared with cells in static culture. *P < 0.05.
