20X M2 Salts	50 ml
0.5 M MgSO4	1 ml
20% Glucose	10 ml	Substitute for H2O in M2
Ferrous Sulfate Chelate Solution	1 ml
0.1 M CaCl2	5 ml	Add last to avoid precipitation
H2O	Fill up to 1 L
Sterile filter with 0.22 µM filter