Figure 4.

Purification of membrane-associated bovine PDE6. Coomassie-stained SDS-PAGE, generated in 1978.⁷ PDE6 purification steps: (1) retina lysate; (2) 30% sucrose 4K supernatant; (3) ROS membranes before sucrose gradient; (4) ROS membranes after sucrose gradient—rhodopsin, 80K protein (transducin), and PDE6αβ are major constituents; (5) hypotonic supernatant; (6) purified PDE6αβ after DEAE column chromatography. S, size markers (bacterial RNA polymerase subunits, bovine serum albumin, and lysozyme).