Figure 3. IκB-ζ-deficient T cells show high levels of IFN-γ expression in the presence of TGF-β.

(A) ELISA for IFN-γ in the supernatants of naïve CD4⁺ T cells from Nfkbiz^flox/flox (Control) and Nfkbiz^flox/floxLck-Cre (cKO) mice that were cultured for 72 hours. Data shown represent means ± sem (n = 3). (B) Flow cytometric analysis of IFN-γ expression in CD4⁺ T cells from control and cKO mice, cultured for 72 hours in the presence or absence of TGF-β1. Data are representative of 4 independent experiments. (C) Percent reduction of IFN-γ expression in CD4⁺ cells under the TGF-β1 condition compared with the non-TGF-β1 condition in B. Data shown represent the means ± sem (n = 4). (D) Flow cytometric analysis of FOXP3 expression in CD4⁺ T cells from control and cKO mice, cultured for 72 hours in the presence or absence of TGF-β1. Data are representative at least 3 independent experiments. (E) Expression of Tbx21 in cultured naive CD4⁺ T cells from control and cKO mice for 24 hours. Data shown represent means ± sem (n = 3). *P < 0.05.