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. 2015 Jul 16;6(8):2908–2922. doi: 10.1364/BOE.6.002908

Table 3. List of Fluids; labeling: AAAAmmdd where ¨mm¨ is the month and ¨dd¨ is the day of creation.

Fluid label Part Number Origin Description Refr. Index
Bact1202A XL1 blue with contamination CBS E.coli bacteria grown for 5 hrs and transferred to Urine1202. Note that the culture was contaminated before incubation with an unknown bacteria measured to have a lower concentration then E.coli and probably a gram positive species. 1.32276
BSA0917 A0281-250 mg S-A Albumin from bovine serum (100 μg/ml). 2 mg of bovine serum albumin was dissolved in 2 ml of PBS and stored in one vial of 2 ml (1 mg/ml) at 4° C. On the day of use, 100 μl from the BSA vial is diluted in 900 μl of PBSG0917 resulting in a 100 μg/ml solution for use in the experiments. N/A
BSA1202 A0281-250 mg S-A Albumin from Bovine Serum (100 μg/ml). This batch mixed from 100 μl of PBS with 900 μl of PBSG1124. Same protocol as BSA0917. N/A
DIH2O D11931 Barnstead Ultra pure water, 18.2 MΩ-cm, < 1CFU/ml, < 1μM/ml (theoretical RI = 1.3206) 1.31977
ECOLI0917 XL1 blue CBS E.coli bacteria grown for 4.3 hrs then transferred to PBSG0917. 1.32854
ECOLI0924 XL1 blue CBS E.coli bacteria grown for 4.3 hrs then transferred to Urine0924. N/A
ECOLI1117 XL1 blue CBS E.coli bacteria grown for 6 hrs, then transferred to Urine1120 and concentration measured with plate culture. N/A
ECOLI1126 XL1 blue CBS E.coli bacteria grown for 7 hrs then transferred to Urine1126. N/A
GNeg AB41202 ABCAM 50 μg/ml of gram negative antibody in solution of PBSG0715. 100 μl of gram negative antibody was diluted in 3900 μl of PBSG0715 and stored in 10 vials of 0.4 ml (50 μg/ml) at 4° C. N/A
GPos AB20344 ABCAM 50 μg/ml of gram positive antibody in solution of PBSG0917. 0.5 ml of gram positive antibodies was diluted in 0.5 ml of PBS and stored in 2 vial of 0.5 ml (50 μg/ml) at 4° C. N/A
GProt0715 P4689-1MG S-A 1 mg of protein G (immunoglobulin-binding protein expressed in group C and G Streptococcal bacteria) was dissolved in 4 ml of PBSG0715 solution and stored in 4 vials of 1 ml (0.25 mg/ml) by freezing at −20° C. On the day of use, a protein G vial was thawed and 200 μL diluted in 800 μL of filtered PBSG solution resulting in a 50 μg/ml solution used to functionalize the bare gold surface of a waveguide with a monolayer of protein G. N/A
HKECOL1001 XL1 blue CBS E.coli bacteria grown for 7 hrs in LB broth, transferred to PBS solution, then heat killed in oven at 80° C for 60 min. N/A
HKECOL1008 XL1 blue CBS HKECOL1001 bacteria transferred to Urine1008 by spinning at 3000 RPM for 7 min. N/A
LB Broth L3022 S-A Mix one pouch in 500 ml of DIH2O. N/A
PBS P-5368 S-A Mix one pouch in 1L DIH2O ml (theoretical RI = 1.3329). 1.32351
PBSG0715 49767-250 ml S-A 66.66 g of glycerol in 500 ml of PBS. 1.33425
PBSG0917 49767-250 ml S-A 33.33 g of glycerol in 500 ml of PBS. 1.32854
PBSG1124 49767-250 ml S-A PBSG0917 sterilized in a microwave oven for 20 min. Final concentration: approximately 10 g/100 ml. 1.33152
SDS 71725-50G S-A 5 g of sodium dodecyl sulfate in 1L of DIH2O. N/A
SEPI0917 ATCC 12228 CBS S.epi bacteria grown for 4.3 hrs then transferred to PBSG0917. 1.32854
SEPI0924 ATCC 12228 CBS S.epi bacteria grown for 4.3 hrs then transferred to Urine0924. N/A
SEPI1117 ATCC 12228 CBS S.epi bacteria grown for 6 hrs then concentration measured with plate culture. N/A
SEPI1126 ATCC 12228 CBS S.epi bacteria grown for 18 hrs then transferred to Urine1126. N/A
SEPI1203 ATCC 12228 CBS S.epi bacteria grown for 17 hrs then transferred to Urine1202. 1.32276
Urine0820A Human anon. First urine of the day collected on 20 Aug. 2014. Donor collected urine in the morning after fasting for 12 hrs. 1.32991
Urine0820B Human anon. 2nd urine of the day collected on 20 Aug. 2014. Urine collected after drinking 1L of water and waiting one hour. 1.32798
Urine0924 Human anon. 2nd urine of the day collected on 24 Sep. 2014 (as above). N/A
Urine1126 Human anon. 2nd urine of the day collected on 26 Nov. 2014 (as above). N/A
Urine1202 Human anon. 2nd urine of the day collected on 2 Dec. 2014 (as above). 1.32276