Bact1202A |
XL1 blue with contamination |
CBS |
E.coli bacteria grown for 5 hrs and transferred to Urine1202. Note that the culture was contaminated before incubation with an unknown bacteria measured to have a lower concentration then E.coli and probably a gram positive species. |
1.32276 |
BSA0917 |
A0281-250 mg |
S-A |
Albumin from bovine serum (100 μg/ml). 2 mg of bovine serum albumin was dissolved in 2 ml of PBS and stored in one vial of 2 ml (1 mg/ml) at 4° C. On the day of use, 100 μl from the BSA vial is diluted in 900 μl of PBSG0917 resulting in a 100 μg/ml solution for use in the experiments. |
N/A |
BSA1202 |
A0281-250 mg |
S-A |
Albumin from Bovine Serum (100 μg/ml). This batch mixed from 100 μl of PBS with 900 μl of PBSG1124. Same protocol as BSA0917. |
N/A |
DIH2O |
D11931 |
Barnstead |
Ultra pure water, 18.2 MΩ-cm, < 1CFU/ml, < 1μM/ml (theoretical RI = 1.3206) |
1.31977 |
ECOLI0917 |
XL1 blue |
CBS |
E.coli bacteria grown for 4.3 hrs then transferred to PBSG0917. |
1.32854 |
ECOLI0924 |
XL1 blue |
CBS |
E.coli bacteria grown for 4.3 hrs then transferred to Urine0924. |
N/A |
ECOLI1117 |
XL1 blue |
CBS |
E.coli bacteria grown for 6 hrs, then transferred to Urine1120 and concentration measured with plate culture. |
N/A |
ECOLI1126 |
XL1 blue |
CBS |
E.coli bacteria grown for 7 hrs then transferred to Urine1126. |
N/A |
GNeg |
AB41202 |
ABCAM |
50 μg/ml of gram negative antibody in solution of PBSG0715. 100 μl of gram negative antibody was diluted in 3900 μl of PBSG0715 and stored in 10 vials of 0.4 ml (50 μg/ml) at 4° C. |
N/A |
GPos |
AB20344 |
ABCAM |
50 μg/ml of gram positive antibody in solution of PBSG0917. 0.5 ml of gram positive antibodies was diluted in 0.5 ml of PBS and stored in 2 vial of 0.5 ml (50 μg/ml) at 4° C. |
N/A |
GProt0715 |
P4689-1MG |
S-A |
1 mg of protein G (immunoglobulin-binding protein expressed in group C and G Streptococcal bacteria) was dissolved in 4 ml of PBSG0715 solution and stored in 4 vials of 1 ml (0.25 mg/ml) by freezing at −20° C. On the day of use, a protein G vial was thawed and 200 μL diluted in 800 μL of filtered PBSG solution resulting in a 50 μg/ml solution used to functionalize the bare gold surface of a waveguide with a monolayer of protein G. |
N/A |
HKECOL1001 |
XL1 blue |
CBS |
E.coli bacteria grown for 7 hrs in LB broth, transferred to PBS solution, then heat killed in oven at 80° C for 60 min. |
N/A |
HKECOL1008 |
XL1 blue |
CBS |
HKECOL1001 bacteria transferred to Urine1008 by spinning at 3000 RPM for 7 min. |
N/A |
LB Broth |
L3022 |
S-A |
Mix one pouch in 500 ml of DIH2O. |
N/A |
PBS |
P-5368 |
S-A |
Mix one pouch in 1L DIH2O ml (theoretical RI = 1.3329). |
1.32351 |
PBSG0715 |
49767-250 ml |
S-A |
66.66 g of glycerol in 500 ml of PBS. |
1.33425 |
PBSG0917 |
49767-250 ml |
S-A |
33.33 g of glycerol in 500 ml of PBS. |
1.32854 |
PBSG1124 |
49767-250 ml |
S-A |
PBSG0917 sterilized in a microwave oven for 20 min. Final concentration: approximately 10 g/100 ml. |
1.33152 |
SDS |
71725-50G |
S-A |
5 g of sodium dodecyl sulfate in 1L of DIH2O. |
N/A |
SEPI0917 |
ATCC 12228 |
CBS |
S.epi bacteria grown for 4.3 hrs then transferred to PBSG0917. |
1.32854 |
SEPI0924 |
ATCC 12228 |
CBS |
S.epi bacteria grown for 4.3 hrs then transferred to Urine0924. |
N/A |
SEPI1117 |
ATCC 12228 |
CBS |
S.epi bacteria grown for 6 hrs then concentration measured with plate culture. |
N/A |
SEPI1126 |
ATCC 12228 |
CBS |
S.epi bacteria grown for 18 hrs then transferred to Urine1126. |
N/A |
SEPI1203 |
ATCC 12228 |
CBS |
S.epi bacteria grown for 17 hrs then transferred to Urine1202. |
1.32276 |
Urine0820A |
Human |
anon. |
First urine of the day collected on 20 Aug. 2014. Donor collected urine in the morning after fasting for 12 hrs. |
1.32991 |
Urine0820B |
Human |
anon. |
2nd urine of the day collected on 20 Aug. 2014. Urine collected after drinking 1L of water and waiting one hour. |
1.32798 |
Urine0924 |
Human |
anon. |
2nd urine of the day collected on 24 Sep. 2014 (as above). |
N/A |
Urine1126 |
Human |
anon. |
2nd urine of the day collected on 26 Nov. 2014 (as above). |
N/A |
Urine1202 |
Human |
anon. |
2nd urine of the day collected on 2 Dec. 2014 (as above). |
1.32276 |