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Transwell assay of migration of HUVECs.
Notes: Cells in five unrepeated visual fields were counted. All experiments were performed in three repetitions. Data are mean ± SEM. *P<0.05 vs control, #P<0.05 vs TGF-β, £P<0.05 vs TGF-β + RLX (200 ng·mL−1).
Abbreviations: HUVECs, human umbilical vein endothelial cells; TGF-β, transforming growth factor β; RLX, relaxin; DAPT, N-[N-(3,5-difluorophenacetyl)-1-alanyl]-S-Phenylglycine t-butyl ester; SEM, standard error of the mean.
