Figure 7.

Dual immunofluorescence staining of CD31 and vimentin to evaluate the EndMT in HUVECs.

Notes: CD31 demonstrated red fluorescence and vimentin demonstrated green fluorescence (magnification ×400). All experiments were performed in three repetitions. Data are mean ± SEM. **P<0.01 vs control, ^##P<0.01 vs TGF-β, ^££P<0.01 vs TGF-β + RLX (200 ng·mL⁻¹).

Abbreviations: RLX, relaxin; TGF-β, transforming growth factor β; HUVECs, human umbilical vein endothelial cells; EndMT, endothelial–mesenchymal transition; DAPT, N-[N-(3,5-difluorophenacetyl)-1-alanyl]-S-Phenylglycine t-butyl ester; SEM, standard error of the mean.