Figure 9.

Western blot analysis of Notch cell signaling pathway.

Notes: The expression of Notch-1 and its downstream proteins HES-1 and Jagged-1 were examined, and the inhibitor of Notch-1 DAPT was added. Blank control; negative control (DAPT); TGF-β-induced group; TGF-β + RLX (200 ng·mL⁻¹); TGF-β + RLX (200 ng·mL⁻¹) + DAPT; all experiments were performed in three repetitions. Data are mean ± SEM. **P<0.01 vs control, ^##P<0.01 vs TGF-β, ^&&P<0.01 vs TGF-β + RLX (200 ng·mL⁻¹).

Abbreviations: RLX, relaxin; TGF-β, transforming growth factor β; DAPT, N-[N-(3,5-difluorophenacetyl)-1-alanyl]-S-Phenylglycine t-butyl ester; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; SEM, standard error of the mean.