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. 2015 Apr 17;(98):52685. doi: 10.3791/52685
Environmental sample Bacterial DNA concentration (ng/ml)* A260/280 A260/230 DNA concentration (ng/µl) before size selection, volume: 25 μl DNA concentration (ng/µl) after size selection, volume: 25 μl
1 0.11 (0.16) 1.8 1.4 27 1.2
2 0.11 (0.20) 1.8 1.3 27.4 3.3
3 0.10 (0.37) 1.8 1.6 19.5 3.4
4 0.04 (0.04) 1.5 0.5 24.4 1.6
5 0.11 (0.13) 1.7 1.0 26.1 1.1
6 0.05 (0.03) 1.5 0.7 15.2 0.4
7 0.01 (0.01) 1.4 0.3 15.1 0.3
8 0.03 (0.05) 1.6 0.7 17 0.9
*Values indicate dsDNA concentration using a high sensitivity fluorescent nucleic acid quantitation assay. Number in parentheses represents concentration using a microvolume spectrophotometer.