Figure 5.

L. reuteri treatment prevents diabetic suppression of Wnt10b in bone. A, Expression of Wnt10b was measured in the tibia bone in control and diabetic wild-type and Wnt10b TG mice using real-time PCR. HPRT was used as a housekeeping gene to normalize the RNA expression. B, Representative images of Wnt10b immunostaining in the local area of osteoblasts and bone marrow of the femur metaphysis of wild-type control (C) and diabetic (D) mice 4 weeks after STZ injection. Magnification, ×40. Arrows point to Wnt10b-positive cells. C, Bar graph represents the number of Wnt10b-positive cells per square millimeter of bone marrow. Bars represent averages ± SE (n = 7–8 per group). *, P < .05.