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. 2015 Jul 2;156(9):3169–3182. doi: 10.1210/EN.2015-1308

Figure 7.

Figure 7.

L. reuteri rescues the TNF-α–induced down-regulation of Wnt10b in osteoblasts. A, Expression of Wnt10b was measured in MC3T3–E1 osteoblasts cocultured with control (C) and diabetic (D) bone marrow for 24 hours. B, Wnt10b, OC, and FABP4 mRNA levels (relative to HPRT levels) were measured in MC3T3–E1 cells cultured for 0 to 21 days with (C) and without (T) TNF-α treatment. Cells were plated at 10 000 cells/mL at day 0, and TNF-α was added 24 hours before harvest. Gene expression was measured using real-time PCR. C, MC3T3 osteoblasts were transfected with a TCF/LEF reporter. After 24 hours, cells were treated with TNF-α and luciferase activity measured 24 hours later. RLU, relative light unit. D, Confluent MC3T3 osteoblasts were treated with the supernatant/medium of an L. reuteri (LR sup) culture or with media alone (vehicle). After 1 hour of pretreatment, cells were treated with TNF-α at 10 ng/mL. Cells were harvested 24 hours later. Wnt10b expression was examined using real-time PCR. Values represent averages ± SE (n = >6 per group). *, P < .05.