CCR6-positive leukocytes are present in human IVF samples. A, Number of CD45+ cells, CCR6+ cells, and double-positive (CD45+CCR6+) cells. The approach to determine these percentages is illustrated in panels B–D. Representative figure of an unstained IVF sample and one in which the gates were set (B) are shown. An IVF sample that was stained for only CD45 (C), stained for only CCR6 (D), or a representative patient sample (E) is also shown.