Fig. 1. DKK-1 expression in Hep3B, Huh7, and 293 cells. (A) RT-PCR analysis showed high DKK-1 mRNA expression in Hep3B cells, low in 293 cells, and none in the negative control. (B) The DKK-1 concentration secreted in the culture media, determined by ELISA, was high in Hep3B cells, moderate in Huh7 cells, and low in 293 cells. (C) Following DKK-1 transfection, luciferase assays revealed that the β-catenin transcriptional activity was induced in 293 cells. ^*p<0.01. DKK-1, dickkopf-1.