Fig. 4. TRESK is expressed exclusively in neurons in the spinal dorsal horn 14 days after spinal nerve ligation (SNL). Double immunofluorescence staining in the ipsilateral lumbar spinal dorsal horn of TRESK (red; A and E) and NeuN, a neuronal marker (green; B) or GFAP, an astrocyte marker (green; F); TRESK was mainly located in neurons, but not astrocytes 14 days after SNL (C, D, G, H). Scale bar=20 µm. TRESK, TWIK-related spinal cord K⁺; GFAP, glial fibrillary acidic protein; DAPI, 40,6-diamidino-2-phenylindole.