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Survival of BtnKO1 and BtnKO2 strain litters. Litters were adjusted to eight pups on day 1 of lactation, and the mothers were either not milked (A and B) or milked (C) with a hand-held vacuum device on days 5, 10, 15, and 20 of lactation (n = 5 animals per genotype). Btn1a1+/+ (filled squares), Btn1a1+/- (open squares), and Btn1a1-/- (filled triangles) are shown.
